In Vitro Evaluation
Antioxidant test (DPPH)
This is a method of evaluating the DPPH radical elimination. DPPH is a purple, stable free radical substance that, when it reacts with antioxidants, receives hydrogen and is reduced and decolorized. This test evaluates the DPPH radical elimination of cosmetic raw materials and natural products based on this principle.
Test period 4 weeks
Antioxidant test (ABTS)
This is a method of evaluating the ABTS radical elimination. It evaluates the ABTS radical elimination of cosmetic raw materials and natural products, based on the principle of decolorization reaction, wherein properties are lost due to a reaction with antioxidants after the generation of ABTS free radicals.
Test period 4 weeks
Antioxidant test (SOD)
Superoxide dismutase (SOD) is a representative antioxidant enzyme and is evaluated by measuring the SOD activity of the sample.
Test period 4 weeks
Measurement test for total polyphenol content
This is a method of measuring the polyphenol content that removes active oxygen. It is evaluated through colorimetric quantification of reducing compounds produced by phenolic substances reacting with the folin-ciocalteau reagents.
Test period 4 weeks
Evaluation of ORAC value
This measures the degree to which a sample inhibits the oxidation of fluorescent labeled substances to non-fluorescent substances by ROO• (peroxyl radicals).
Test period 4 weeks
ROS inhibition test
Evaluates intracellular ROS (reactive oxygen species) occurrence through DCFDA (dichlorofluorescin diacetate) fluorescence staining.
Test period 6 weeks
Cytotoxicity test
This evaluates the cytotoxicity of cosmetic raw materials and natural products, using methods such as MTT, WST-1, ALAMAR Blue assay, etc.
Test period 4 weeks
Cell migration test
This is a method of evaluating the effect of a sample on cell migration. It evaluates the cell migration by putting in an insert and generating a wound after culturing a single layer of cells.
Test period 6 weeks
Cell viability test using dermal papilla cells
The MTT method is used to determine the cell viability or the dermal papilla cells (the cells related to hair loss) on which to investigate the effect of the sample on the cell viability.
Test period 4 weeks
Test of factors related to hair loss
This evaluates the mRNA expression of hair loss-related factors using the RT-qPCR method.
Test period 8 weeks
Collagen production test
Perfom a collagen synthesis assay to identify whether collagen production is increased when the sample is applied to human dermal fibroblasts in accordance with the Guideline for Efficacy Evaluation of Functional Cosmetics for Wrinkle Improvement published by the Ministry of Food and Drug Safety
Test period 6 weeks
Collagenase activity inhibition test
This evaluates whether the sample inhibits the activity of collagenase in human dermal fibroblast cells, in accordance with the Ministry of Food and Drug Safety’s “Guideline for Efficacy Evaluation of Functional Cosmetics that Help to Improve Skin Wrinkles - Intracellular Collagenase Activity Inhibition Test.”
Test period 8 weeks
Elastase activity inhibition test
This evaluates the degree to which samples inhibit elastase activity using human dermal fibroblast cells, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help to Improve Skin Wrinkles - Elastase Activity Inhibition Test.”
Test period 6 weeks
Elastin production test
This evaluates elastin extracted from cells with a quantitative dye-binding method.
Test period 6 weeks
Melanin production inhibition test
This evaluates whether the sample inhibits melanin production after stimulating B16F10 cells with melanin production-inducing substances, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - Melanogenesis Inhibition Test."
Test period 8 weeks
Tyrosinase activity inhibition test
This evaluates tyrosinase activity inhibition through an enzyme-substrate reaction using mushroom tyrosinase and L-tyrosine, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - DOPA Oxidation Inhibition Test."
Test period 4 weeks
Tyrosinase mRNA Inhibition Assay
Perform a tyrosinase mRNA inhibition assay to identify tyrosinase mRNA expression level in cells in compliance with the Guideline for Efficacy Evaluation of Functional Cosmetics for Skin Lightening published by the Ministry of Food and Drug Safety
Test period 8 weeks
DOPA oxidation reaction inhibition test
This evaluates DOPA oxidation reaction inhibition through an enzyme-substrate reaction using mushroom tyrosinase and L-DOPA, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - DOPA Oxidation Inhibition Test."
Test period 4 weeks
Nitric oxide (NO) measurement test
This evaluates whether the sample has the effect of inhibiting the production of NO in NO production-induced macrophages.
Test period 4 weeks
Test for Expression Level of Inflammatory Gene mRNA Attributed to Exposure to Particulate Matter
Conduct RT–qPCR experiments to identify how the sample affects the expression level of inflammatory gene mRNA attributed to exposure to particulate matter using keratinocytes
Test period 8 weeks
TARC inhibition test
This evaluates the effect of inhibiting the generation of TARC, an atopy-related indicator.
Test period 6 weeks
ELISA assay
This is an enzyme-linked immunosorbent assay. It evaluates the color reaction using the enzyme substrates and specific binding reactions between antigens and antibodies.
Test period 8 weeks
RT-PCR & RT-qPCR
This evaluates the mRNA expression of gene related to inflammation, skin aging, the skin barrier and skin moisturization using the RT-PCR and RT-qPCR methods.
Test period 8 weeks
Western blot
This evaluates the expression of proteins related to inflammation, skin aging, the skin barrier and skin moisturization at the intracellular protein level.
Test period 8 weeks
Immunofluorescence staining test
This evaluates imaging by labeling fluorescent staining on an antibody that exhibits specific binding to a particular antigen or compound.
Test period 4 weeks
3T3 NRU phototoxicity test
This evaluates potential phototoxicity induced by chemical substances caused after exposure to light through an in vitro 3T3 NRU (neutral red uptake) phototoxicity test, in accordance with the provisions of the Ministry of Food and Drug Safety’s “Guidelines for Alternative Animal Testing Methods for Cosmetic Toxicity Testing (I).”
Test period 8 weeks
Hair loss testing using micro-patterned 3D hair follicle organoids
Evaluate the pillar growth of keratinocytes by co-culturing them with dermal papilla cells in a specially designed pattern plate
Test period 6 weeks
Short Time Exposure (STE) test for eye irritation using Statens Seruminstitut Rabbit Cornea(SIRC) cell line as an alternative to animal testing
Perform UN GHS classification based on the cell viability of the sample using the SIRC cells
Test period 3 weeks