In Vitro Evaluation
Antioxidant test (DPPH)
This is a method of evaluating the DPPH radical elimination. DPPH is a purple, stable free radical substance that, when it reacts with antioxidants, receives hydrogen and is reduced and decolorized. This test evaluates the DPPH radical elimination of cosmetic raw materials and natural products based on this principle.
Test period 4 weeks
Antioxidant test (ABTS)
This is a method of evaluating the ABTS radical elimination. It evaluates the ABTS radical elimination of cosmetic raw materials and natural products, based on the principle of decolorization reaction, wherein properties are lost due to a reaction with antioxidants after the generation of ABTS free radicals.
Test period 4 weeks
Antioxidant test (SOD)
Superoxide dismutase (SOD) is a representative antioxidant enzyme and is evaluated by measuring the SOD activity of the sample.
Test period 6 weeks
Measurement test for total polyphenol content
This is a method of measuring the polyphenol content that removes active oxygen. It is evaluated through colorimetric quantification of reducing compounds produced by phenolic substances reacting with the folin-ciocalteau reagents.
Test period 4 weeks
Evaluation of ORAC value
This measures the degree to which a sample inhibits the oxidation of fluorescent labeled substances to non-fluorescent substances by ROO• (peroxyl radicals).
Test period 6 weeks
ROS inhibition test
Evaluates intracellular ROS (reactive oxygen species) occurrence through DCFDA (dichlorofluorescin diacetate) fluorescence staining.
Test period 6 weeks
Cytotoxicity test
This evaluates the cytotoxicity of cosmetic raw materials and natural products, using methods such as MTT, WST-1, ALAMAR Blue assay, etc.
Test period 6 weeks
Cell migration test
This is a method of evaluating the effect of a sample on cell migration. It evaluates the cell migration by putting in an insert and generating a wound after culturing a single layer of cells.
Test period 6 weeks
Cell viability test using dermal papilla cells
The MTT method is used to determine the cell viability or the dermal papilla cells (the cells related to hair loss) on which to investigate the effect of the sample on the cell viability.
Test period 6 weeks
Test of factors related to hair loss other than 5A-reductase
This evaluates the mRNA expression of hair loss-related factors using the RT-QPCR method.
Test period 8 weeks
Collagen production test
HU in accordance with the Ministry of Food and Drug Safety’s “Guideline for Efficacy Evaluation of Functional Cosmetics that Help to Improve Skin Wrinkles - Intracellular Collagen Production Test."
Test period 6 weeks
Collagenase activity inhibition test
This evaluates whether the sample inhibits the activity of collagenase in human dermal fibroblast cells, in accordance with the Ministry of Food and Drug Safety’s “Guideline for Efficacy Evaluation of Functional Cosmetics that Help to Improve Skin Wrinkles - Intracellular Collagenase Activity Inhibition Test.”
Test period 8 weeks
Elastase activity inhibition test
This evaluates the degree to which samples inhibit elastase activity using human dermal fibroblast cells, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help to Improve Skin Wrinkles - Elastase Activity Inhibition Test.”
Test period 6 weeks
Elastin production test
This evaluates elastin extracted from cells with a quantitative dye-binding method.
Test period 6 weeks
Melanin production inhibition test
This evaluates whether the sample inhibits melanin production after stimulating B16F10 cells with melanin production-inducing substances, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - Melanogenesis Inhibition Test."
Test period 8 weeks
Tyrosinase activity inhibition test
This evaluates tyrosinase activity inhibition through an enzyme-substrate reaction using mushroom tyrosinase and L-tyrosine, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - DOPA Oxidation Inhibition Test."
Test period 4 weeks
Tyrosinase activity inhibition test
This evaluates intracellular tyrosine mRNA expression in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - Tyrosinase mRNA Expression Inhibition Test."
Test period 8 weeks
DOPA oxidation reaction inhibition test
This evaluates DOPA oxidation reaction inhibition through an enzyme-substrate reaction using mushroom tyrosinase and L-DOPA, in accordance with the Ministry of Food and Drug Safety’s “Guideline for the Efficacy Evaluation of Functional Cosmetics that Help with Skin Whitening - DOPA Oxidation Inhibition Test."
Test period 4 weeks
Nitric oxide (NO) measurement test
This evaluates whether the sample has the effect of inhibiting the production of NO in NO production-induced macrophages.
Test period 6 weeks
Measurement test of the inflammation and mRNA expression level of the skin barrier factor caused by fine dust
This uses the RT-QPCR method to determine how samples using skin keratinocytes affect the mRNA expression levels of genes related to inflammation and the skin barrier caused by fine dust.
Test period 8 weeks
TARC inhibition test
This evaluates the effect of inhibiting the generation of TARC, an atopy-related indicator.
Test period 6 weeks
ELISA ASSAY
This is an enzyme-linked immunosorbent assay. It evaluates the color reaction using the enzyme substrates and specific binding reactions between antigens and antibodies.
Test period 8 weeks
LUCIFERASE ASSAY
This evaluates the promoter activation and gene expression level of specific genes using luciferase, an enzyme that uses luciferin as a substrate to catalyze light (luminescence).
Test period 8 weeks
RT-PCR & RT-QPCR
This evaluates the mRNA expression of gene related to inflammation, skin aging, the skin barrier and skin moisturization using the RT-PCR and RT-QPCR methods.
Test period 8 weeks
WESTERN BLOT
This evaluates the expression of proteins related to inflammation, skin aging, the skin barrier and skin moisturization at the intracellular protein level.
Test period 8 weeks
Immunofluorescence staining test
This evaluates imaging by labeling fluorescent staining on an antibody that exhibits specific binding to a particular antigen or compound.
Test period 8 weeks
3T3 NRU phototoxicity test
This evaluates potential phototoxicity induced by chemical substances caused after exposure to light through an in vitro 3T3 NRU (neutral red uptake) phototoxicity test, in accordance with the provisions of the Ministry of Food and Drug Safety’s “Guidelines for Alternative Animal Testing Methods for Cosmetic Toxicity Testing (I).”
Test period 8 weeks
Alternative skin irritation test using artificial skin (non-GLP)
This proceeds with an alternative skin irritation test using an artificial skin model (reconstructed human epidermis) in accordance with the OECD guidelines (TG 439).
Test period 12 weeks